KLHL22

kelch like family member 22 | FLJ14360, KELCHL

Enables 14-3-3 protein binding activity and ubiquitin-like ligase-substrate adaptor activity. Involved in several processes, including cellular response to L-leucine; mitotic spindle assembly checkpoint signaling; and negative regulation of metabolic process. Located in several cellular components, including intercellular bridge; lysosome; and microtubule cytoskeleton. Part of Cul3-RING ubiquitin ligase complex. [provided by Alliance of Genome Resources, Jul 2025]

Member of: DE-3 DE-3.4 Developmental clusters: GC7

Biological processes 48 terms

14-3-3 protein binding (GO:0071889)Cul3-RING ubiquitin ligase complex (GO:0031463)Cul3-RING ubiquitin ligase complex (GO:0031463)Cul3-RING ubiquitin ligase complex (GO:0031463)cell division (GO:0051301)cell division (GO:0051301)cellular response to L-leucine (GO:0071233)cellular response to amino acid stimulus (GO:0071230)cellular response to amino acid stimulus (GO:0071230)centrosome (GO:0005813)centrosome (GO:0005813)cytoplasm (GO:0005737)cytoplasm (GO:0005737)cytosol (GO:0005829)cytosol (GO:0005829)cytosol (GO:0005829)cytosol (GO:0005829)intercellular bridge (GO:0045171)lysosome (GO:0005764)lysosome (GO:0005764)mitotic sister chromatid segregation (GO:0000070)mitotic spindle (GO:0072686)mitotic spindle (GO:0072686)mitotic spindle assembly checkpoint signaling (GO:0007094)negative regulation of autophagy (GO:0010507)negative regulation of type I interferon production (GO:0032480)nucleus (GO:0005634)nucleus (GO:0005634)nucleus (GO:0005634)polar microtubule (GO:0005827)polar microtubule (GO:0005827)positive regulation of T cell activation (GO:0050870)positive regulation of T cell mediated immune response to tumor cell (GO:0002842)positive regulation of TORC1 signaling (GO:1904263)positive regulation of TORC1 signaling (GO:1904263)positive regulation of TORC1 signaling (GO:1904263)positive regulation of cell growth (GO:0030307)proteasome-mediated ubiquitin-dependent protein catabolic process (GO:0043161)proteasome-mediated ubiquitin-dependent protein catabolic process (GO:0043161)proteasome-mediated ubiquitin-dependent protein catabolic process (GO:0043161)protein binding (GO:0005515)protein monoubiquitination (GO:0006513)protein monoubiquitination (GO:0006513)protein ubiquitination (GO:0016567)spindle (GO:0005819)ubiquitin-dependent protein catabolic process (GO:0006511)ubiquitin-like ligase-substrate adaptor activity (GO:1990756)ubiquitin-like ligase-substrate adaptor activity (GO:1990756)

Expression (TPM)

TFs regulating KLHL22 0 TFs

Transcription factors with Perturb-seq knockdown data for KLHL22. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = KLHL22 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

TF	Mean coef	Binding	Outlier	TF→Gene link

Rows:

Elements linked to KLHL22

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of KLHL22, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Element	Dist. to TSS	Link type	TFs
chr22:20,203,393–20,204,086	292.0 kb	Distal (>10kb) Multiome	292
chr22:20,243,636–20,244,300	251.8 kb	Distal (>10kb) Multiome	251
chr22:20,267,373–20,268,700	227.4 kb	Distal (>10kb) Multiome	449
chr22:20,298,366–20,300,076	196.6 kb	Distal (>10kb) Multiome	538
chr22:20,319,527–20,320,815	175.7 kb	Distal (>10kb) Multiome	805
chr22:20,393,744–20,394,682	101.7 kb	Distal (>10kb) Multiome	854
chr22:20,418,190–20,418,662	77.4 kb	Distal (>10kb) Multiome	594
chr22:20,431,567–20,432,135	64.0 kb	Distal (>10kb) Multiome	135
chr22:20,437,361–20,438,451	57.8 kb	Distal (>10kb) Multiome	393
chr22:20,495,029–20,496,932	57 bp	At TSS Multiome	1018
chr22:20,507,376–20,507,935	11.8 kb	Distal (>10kb) Multiome	894
chr22:20,606,015–20,606,540	110.6 kb	Distal (>10kb) Multiome	411
chr22:20,702,218–20,703,674	207.2 kb	Distal (>10kb) Multiome	438

Genome Browser

Genomic view of the KLHL22 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.