Transcription factors with Perturb-seq knockdown data for KCNQ5-DT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = KCNQ5-DT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of KCNQ5-DT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:72,614,017–72,614,200 | 7.9 kb | Proximal (<10kb) | 3 | |
| chr6:72,619,868–72,623,447 | at TSS | At TSS | 709 | |
| chr6:72,624,669–72,624,867 | 2.6 kb | Proximal (<10kb) | 138 | |
| chr6:72,626,097–72,626,496 | 4.0 kb | Proximal (<10kb) | 92 | |
| chr6:72,627,664–72,629,016 | 5.6 kb | Proximal (<10kb) | 149 |
Genomic view of the KCNQ5-DT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.