This gene encodes a member of the HIN-200 (hematopoietic interferon-inducible nuclear antigens with 200 amino acid repeats) family of cytokines. The encoded protein contains domains involved in DNA binding, transcriptional regulation, and protein-protein interactions. The protein localizes to the nucleoplasm and nucleoli, and interacts with p53 and retinoblastoma-1. It modulates p53 function, and inhibits cell growth in the Ras/Raf signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2011]
Transcription factors with Perturb-seq knockdown data for IFI16. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = IFI16 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of IFI16, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:158,149,364–158,150,151 | 850.1 kb | Distal (>10kb) Multiome HiCAR | 421 | |
| chr1:158,998,556–158,999,168 | 798 bp | At TSS | 246 | |
| chr1:158,999,415–159,000,347 | 118 bp | At TSS Multiome | 267 | |
| chr1:159,000,728–159,000,933 | 761 bp | At TSS | 102 | |
| chr1:159,005,384–159,006,879 | 6.0 kb | Proximal (<10kb) Multiome | 342 | |
| chr1:159,171,210–159,173,452 | 171.7 kb | Distal (>10kb) Multiome | 251 | |
| chr1:159,187,164–159,189,000 | 188.6 kb | Distal (>10kb) Multiome | 228 |
Genomic view of the IFI16 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.