Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the gamma subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. This gene is a candidate gene for periventricular heterotopia. Several alternatively spliced transcript variants of this gene have been described, but only some of their full length natures have been determined. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for IDH3G. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = IDH3G upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of IDH3G, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chrX:153,598,511–153,599,722 | 195.1 kb | Distal (>10kb) Multiome | 459 | |
| chrX:153,609,905–153,610,354 | 184.3 kb | Distal (>10kb) Multiome | 404 | |
| chrX:153,687,144–153,688,247 | 106.6 kb | Distal (>10kb) Multiome | 201 | |
| chrX:153,723,829–153,725,285 | 69.8 kb | Distal (>10kb) Multiome | 620 | |
| chrX:153,726,395–153,727,168 | 67.5 kb | Distal (>10kb) Multiome | 529 | |
| chrX:153,793,808–153,795,045 | 50 bp | At TSS Multiome | 733 | |
| chrX:153,875,415–153,875,856 | 81.3 kb | Distal (>10kb) Multiome | 847 | |
| chrX:153,878,887–153,879,684 | 84.8 kb | Distal (>10kb) Multiome | 235 | |
| chrX:153,927,955–153,928,728 | 134.0 kb | Distal (>10kb) Multiome | 711 | |
| chrX:153,934,763–153,935,658 | 140.8 kb | Distal (>10kb) Multiome | 446 | |
| chrX:153,970,913–153,973,065 | 178.1 kb | Distal (>10kb) Multiome | 857 |
Genomic view of the IDH3G locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.