Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the beta subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Sep 2016]
Transcription factors with Perturb-seq knockdown data for IDH3B. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = IDH3B upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of IDH3B, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr20:2,470,152–2,471,296 | 193.3 kb | Distal (>10kb) Multiome | 1126 | |
| chr20:2,508,648–2,509,663 | 155.1 kb | Distal (>10kb) Multiome | 854 | |
| chr20:2,524,432–2,525,455 | 139.5 kb | Distal (>10kb) Multiome | 724 | |
| chr20:2,536,033–2,536,583 | 127.9 kb | Distal (>10kb) Multiome | 42 | |
| chr20:2,651,802–2,653,286 | 11.6 kb | Distal (>10kb) Multiome | 1060 | |
| chr20:2,658,803–2,659,102 | 5.1 kb | Proximal (<10kb) | 315 | |
| chr20:2,663,925–2,664,409 | 54 bp | At TSS Multiome | 908 | |
| chr20:2,692,159–2,694,013 | 28.4 kb | Distal (>10kb) Multiome | 584 | |
| chr20:2,752,636–2,753,047 | 88.6 kb | Distal (>10kb) Multiome | 395 | |
| chr20:2,800,326–2,801,021 | 136.5 kb | Distal (>10kb) Multiome | 349 | |
| chr20:2,840,006–2,841,330 | 176.5 kb | Distal (>10kb) Multiome | 997 | |
| chr20:2,872,329–2,874,047 | 209.3 kb | Distal (>10kb) Multiome | 980 |
Genomic view of the IDH3B locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.