Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for IDH3A. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = IDH3A upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of IDH3A, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr15:78,077,036–78,077,991 | 53.9 kb | Distal (>10kb) Multiome | 552 | |
| chr15:78,130,851–78,132,005 | 7 bp | At TSS Multiome | 769 | |
| chr15:78,148,893–78,150,142 | 17.8 kb | Distal (>10kb) Multiome | 746 | |
| chr15:78,264,436–78,265,112 | 133.2 kb | Distal (>10kb) Multiome | 241 | |
| chr15:78,299,130–78,299,874 | 168.2 kb | Distal (>10kb) Multiome | 798 | |
| chr15:78,303,744–78,304,214 | 172.4 kb | Distal (>10kb) Multiome | 287 | |
| chr15:78,340,100–78,340,745 | 208.8 kb | Distal (>10kb) Multiome | 194 | |
| chr15:78,348,106–78,348,824 | 217.0 kb | Distal (>10kb) Multiome | 178 | |
| chr15:78,437,554–78,438,897 | 306.7 kb | Distal (>10kb) Multiome | 918 |
Genomic view of the IDH3A locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.