This gene encodes a DnaJ-type co-chaperone and member of the heat shock cognate B (HscB) family of proteins. The encoded protein plays a role in the synthesis of iron-sulfur clusters, protein cofactors that are involved in the redox reactions of mitochondrial electron transport and other processes. Cells in which this gene is knocked down exhibit reduced activity of iron-sulfur cluster-dependent enzymes including succinate dehydrogenase and aconitase. The encoded protein may stimulate the ATPase activity of the mitochondrial stress-70 protein. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
Transcription factors with Perturb-seq knockdown data for HSCB. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = HSCB upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of HSCB, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr22:28,442,238–28,442,891 | 299.6 kb | Distal (>10kb) Multiome | 615 | |
| chr22:28,679,491–28,680,261 | 62.1 kb | Distal (>10kb) Multiome | 374 | |
| chr22:28,736,360–28,736,647 | 5.4 kb | Proximal (<10kb) | 92 | |
| chr22:28,741,336–28,742,751 | 137 bp | At TSS Multiome | 926 | |
| chr22:28,772,518–28,773,373 | 30.7 kb | Distal (>10kb) Multiome | 796 | |
| chr22:28,800,006–28,801,035 | 58.5 kb | Distal (>10kb) Multiome | 829 | |
| chr22:28,882,670–28,884,893 | 141.3 kb | Distal (>10kb) Multiome | 802 | |
| chr22:29,004,535–29,005,249 | 262.9 kb | Distal (>10kb) Multiome | 417 | |
| chr22:29,029,712–29,031,115 | 288.8 kb | Distal (>10kb) Multiome | 773 |
Genomic view of the HSCB locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.