The protein encoded by this gene is a type II integral membrane protein that belongs to the 3-O-sulfotransferases family. These proteins catalyze the addition of sulfate groups at the 3-OH position of glucosamine in heparan sulfate. The substrate specificity of individual members of the family is based on prior modification of the heparan sulfate chain, thus allowing different members of the family to generate binding sites for different proteins on the same heparan sulfate chain. Following treatment with a histone deacetylase inhibitor, expression of this gene is activated in a pancreatic cell line. The increased expression results in promotion of the epithelial-mesenchymal transition. In addition, the modification catalyzed by this protein allows herpes simplex virus membrane fusion and penetration. A very closely related homolog with an almost identical sulfotransferase domain maps less than 1 Mb away. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
Transcription factors with Perturb-seq knockdown data for HS3ST3B1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = HS3ST3B1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of HS3ST3B1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:13,017,268–13,018,649 | 1283.0 kb | Distal (>10kb) Multiome HiCAR | 828 | |
| chr17:13,600,452–13,602,595 | 699.0 kb | Distal (>10kb) Multiome HiCAR | 570 | |
| chr17:14,069,121–14,069,983 | 231.6 kb | Distal (>10kb) Multiome | 810 | |
| chr17:14,297,092–14,298,113 | 3.5 kb | Proximal (<10kb) Multiome | 567 | |
| chr17:14,298,503–14,298,726 | 2.4 kb | Proximal (<10kb) | 87 | |
| chr17:14,299,291–14,299,916 | 1.2 kb | Proximal (<10kb) | 432 | |
| chr17:14,300,397–14,303,082 | 110 bp | At TSS Multiome | 904 | |
| chr17:14,303,513–14,303,964 | 2.4 kb | Proximal (<10kb) | 170 | |
| chr17:14,304,075–14,304,390 | 3.0 kb | Proximal (<10kb) | 143 | |
| chr17:14,308,769–14,310,537 | 8.4 kb | Proximal (<10kb) Multiome | 830 | |
| chr17:14,323,613–14,324,323 | 22.8 kb | Distal (>10kb) Multiome | 110 | |
| chr17:14,331,196–14,332,727 | 30.7 kb | Distal (>10kb) Multiome | 319 | |
| chr17:14,451,133–14,452,617 | 151.0 kb | Distal (>10kb) Multiome | 142 | |
| chr17:14,525,928–14,526,410 | 225.1 kb | Distal (>10kb) Multiome | 57 |
Genomic view of the HS3ST3B1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.