Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. This gene encodes a member of the heparan sulfate biosynthetic enzyme family that transfers sulfate to the 2 position of the iduronic acid residue of heparan sulfate. The disruption of this gene resulted in no kidney formation in knockout embryonic mice, indicating that the absence of this enzyme may interfere with the signaling required for kidney formation. Two alternatively spliced transcript variants that encode different proteins have been found for this gene. [provided by RefSeq, Aug 2008]
Transcription factors with Perturb-seq knockdown data for HS2ST1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = HS2ST1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of HS2ST1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:86,703,797–86,705,777 | 210.1 kb | Distal (>10kb) Multiome | 1030 | |
| chr1:86,755,319–86,756,660 | 158.8 kb | Distal (>10kb) Multiome | 183 | |
| chr1:86,823,769–86,824,525 | 90.7 kb | Distal (>10kb) Multiome | 408 | |
| chr1:86,913,509–86,916,034 | 274 bp | At TSS Multiome | 926 | |
| chr1:87,131,612–87,132,886 | 217.2 kb | Distal (>10kb) Multiome | 494 | |
| chr1:87,133,487–87,134,387 | 219.1 kb | Distal (>10kb) Multiome | 138 | |
| chr1:87,151,430–87,152,405 | 237.3 kb | Distal (>10kb) Multiome | 383 |
Genomic view of the HS2ST1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.