This gene encodes a member of the hook family of coiled-coil proteins, which bind to microtubules and organelles through their N- and C-terminal domains, respectively. The encoded protein localizes to discrete punctuate subcellular structures, and interacts with several members of the Rab GTPase family involved in endocytosis. It is thought to link endocytic membrane trafficking to the microtubule cytoskeleton. Several alternatively spliced transcript variants have been identified, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for HOOK1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = HOOK1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of HOOK1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:58,576,513–58,577,828 | 1237.7 kb | Distal (>10kb) Multiome HiCAR | 406 | |
| chr1:58,699,577–58,700,464 | 1114.8 kb | Distal (>10kb) Multiome HiCAR | 702 | |
| chr1:58,704,094–58,705,219 | 1110.1 kb | Distal (>10kb) Multiome HiCAR | 154 | |
| chr1:58,814,035–58,816,778 | 998.5 kb | Distal (>10kb) Multiome HiCAR | 966 | |
| chr1:58,903,394–58,904,485 | 911.0 kb | Distal (>10kb) Multiome HiCAR | 782 | |
| chr1:59,542,856–59,543,707 | 271.6 kb | Distal (>10kb) Multiome | 210 | |
| chr1:59,769,240–59,769,882 | 45.4 kb | Distal (>10kb) Multiome | 103 | |
| chr1:59,814,141–59,815,752 | 174 bp | At TSS Multiome | 767 | |
| chr1:59,817,713–59,817,934 | 2.8 kb | Proximal (<10kb) | 8 | |
| chr1:60,052,385–60,053,492 | 237.9 kb | Distal (>10kb) Multiome | 188 | |
| chr1:60,073,433–60,074,192 | 258.9 kb | Distal (>10kb) Multiome | 194 | |
| chr1:61,436,457–61,437,346 | 1622.0 kb | Distal (>10kb) Multiome HiCAR | 172 |
Genomic view of the HOOK1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.