Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq, Apr 2009]
Transcription factors with Perturb-seq knockdown data for HK2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = HK2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of HK2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:74,548,706–74,549,795 | 284.7 kb | Distal (>10kb) Multiome | 625 | |
| chr2:74,553,035–74,555,636 | 279.6 kb | Distal (>10kb) Multiome | 768 | |
| chr2:74,647,804–74,648,489 | 185.9 kb | Distal (>10kb) Multiome | 232 | |
| chr2:74,653,808–74,654,908 | 179.9 kb | Distal (>10kb) Multiome | 658 | |
| chr2:74,715,073–74,715,890 | 118.8 kb | Distal (>10kb) Multiome HiCAR | 768 | |
| chr2:74,777,656–74,779,073 | 55.5 kb | Distal (>10kb) Multiome | 426 | |
| chr2:74,816,374–74,817,201 | 17.3 kb | Distal (>10kb) Multiome | 373 | |
| chr2:74,822,732–74,823,334 | 11.2 kb | Distal (>10kb) Multiome | 40 | |
| chr2:74,833,573–74,835,838 | 1.3 kb | Proximal (<10kb) Multiome | 968 | |
| chr2:74,836,514–74,836,751 | 2.4 kb | Proximal (<10kb) | 239 | |
| chr2:74,837,028–74,837,326 | 2.9 kb | Proximal (<10kb) | 96 | |
| chr2:74,919,661–74,920,281 | 85.9 kb | Distal (>10kb) Multiome | 376 | |
| chr2:74,958,221–74,959,258 | 124.6 kb | Distal (>10kb) Multiome | 1096 | |
| chr2:75,055,603–75,056,664 | 222.2 kb | Distal (>10kb) Multiome | 263 |
Genomic view of the HK2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.