This gene encodes an evolutionarily conserved nuclear transport receptor that mediates heat-shock-induced nuclear import of 70 kDa heat-shock proteins (Hsp70s) through interactions with FG-nucleoporins. The protein mediates transport of the ATP form but not the ADP form of Hsp70 proteins under conditions of heat shock stress. Structural analyses demonstrate that the protein forms an asymmetric homodimer and that the N-terminal domain consists of a jelly-roll/beta-sandwich fold structure that contains hydrophobic pockets involved in FG-nucleoporin recognition. Reduction of RNA expression levels in HeLa cells using small interfering RNAs results in inhibition of heat shock-induced nuclear accumulation of Hsp70s, indicating a role for this gene in regulation of Hsp70 nuclear import during heat shock stress. [provided by RefSeq, Apr 2016]
Transcription factors with Perturb-seq knockdown data for HIKESHI. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = HIKESHI upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of HIKESHI, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr11:86,052,796–86,053,429 | 249.1 kb | Distal (>10kb) Multiome | 169 | |
| chr11:86,068,044–86,070,093 | 233.0 kb | Distal (>10kb) Multiome | 1026 | |
| chr11:86,151,187–86,151,901 | 150.7 kb | Distal (>10kb) Multiome | 356 | |
| chr11:86,172,744–86,173,502 | 129.2 kb | Distal (>10kb) Multiome | 240 | |
| chr11:86,244,135–86,246,099 | 57.2 kb | Distal (>10kb) Multiome | 959 | |
| chr11:86,302,015–86,302,729 | 25 bp | At TSS Multiome | 873 | |
| chr11:86,374,442–86,374,974 | 72.6 kb | Distal (>10kb) Multiome | 124 | |
| chr11:86,421,815–86,422,714 | 120.0 kb | Distal (>10kb) Multiome | 141 | |
| chr11:86,422,902–86,424,146 | 121.5 kb | Distal (>10kb) Multiome | 152 | |
| chr11:86,513,467–86,514,411 | 211.7 kb | Distal (>10kb) Multiome | 148 | |
| chr11:86,571,370–86,571,978 | 269.5 kb | Distal (>10kb) Multiome | 310 |
Genomic view of the HIKESHI locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.