Transcription factors with Perturb-seq knockdown data for HEY2-AS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = HEY2-AS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of HEY2-AS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:125,528,427–125,529,436 | 216.8 kb | Distal (>10kb) Multiome HiCAR | 266 | |
| chr6:125,700,629–125,701,546 | 44.5 kb | Distal (>10kb) Multiome HiCAR | 153 | |
| chr6:125,745,345–125,746,068 | 145 bp | At TSS Multiome | 210 | |
| chr6:125,746,584–125,747,315 | 1.3 kb | Proximal (<10kb) Multiome | 391 | |
| chr6:125,747,542–125,750,482 | 3.9 kb | Proximal (<10kb) Multiome | 891 | |
| chr6:125,780,157–125,781,423 | 34.8 kb | Distal (>10kb) Multiome | 1022 | |
| chr6:125,789,677–125,791,851 | 45.2 kb | Distal (>10kb) Multiome | 1017 | |
| chr6:125,867,468–125,867,998 | 122.2 kb | Distal (>10kb) Multiome | 264 | |
| chr6:125,952,889–125,953,903 | 207.9 kb | Distal (>10kb) Multiome | 343 | |
| chr6:125,956,540–125,957,527 | 211.2 kb | Distal (>10kb) Multiome | 1085 | |
| chr6:125,986,066–125,987,257 | 240.9 kb | Distal (>10kb) Multiome | 925 |
Genomic view of the HEY2-AS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.