HERC4 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins (Hochrainer et al., 2005 [PubMed 15676274]).[supplied by OMIM, Mar 2008]
Transcription factors with Perturb-seq knockdown data for HERC4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = HERC4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of HERC4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr10:67,788,290–67,788,969 | 286.7 kb | Distal (>10kb) Multiome | 406 | |
| chr10:67,849,336–67,850,018 | 225.8 kb | Distal (>10kb) Multiome | 751 | |
| chr10:67,861,706–67,862,739 | 213.2 kb | Distal (>10kb) Multiome | 215 | |
| chr10:67,884,003–67,885,448 | 190.6 kb | Distal (>10kb) Multiome | 1061 | |
| chr10:68,074,303–68,075,534 | 128 bp | At TSS Multiome | 860 | |
| chr10:68,208,472–68,208,961 | 133.4 kb | Distal (>10kb) Multiome | 254 | |
| chr10:68,231,028–68,232,253 | 156.2 kb | Distal (>10kb) Multiome | 654 | |
| chr10:68,271,165–68,272,025 | 196.3 kb | Distal (>10kb) Multiome | 177 | |
| chr10:68,331,343–68,333,064 | 257.7 kb | Distal (>10kb) Multiome | 1174 |
Genomic view of the HERC4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.