Transcription factors with Perturb-seq knockdown data for HELLPAR. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = HELLPAR upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of HELLPAR, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:102,061,470–102,062,548 | 135.5 kb | Distal (>10kb) Multiome | 687 | |
| chr12:102,119,615–102,121,249 | 77.4 kb | Distal (>10kb) Multiome | 891 | |
| chr12:102,154,546–102,155,516 | 42.6 kb | Distal (>10kb) Multiome | 90 | |
| chr12:102,201,075–102,201,302 | 3.5 kb | Proximal (<10kb) | 8 | |
| chr12:102,824,128–102,824,965 | 627.0 kb | Distal (>10kb) Multiome HiCAR | 512 |
Genomic view of the HELLPAR locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.