This gene encodes one of two proteins which interact with VP16, a herpes simplex virus protein that initiates virus infection. Both the encoded protein and the original Herpes host cell factor interact with VP16 through a beta-propeller domain. The original Herpes host cell factor, however, is effective at initiating viral infection while the encoded protein is not. Transcripts of varying length due to alternative polyadenylation signals have been described. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for HCFC2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = HCFC2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of HCFC2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:103,825,918–103,826,568 | 238.2 kb | Distal (>10kb) Multiome | 82 | |
| chr12:103,840,241–103,842,325 | 223.2 kb | Distal (>10kb) Multiome | 911 | |
| chr12:103,928,943–103,931,279 | 134.3 kb | Distal (>10kb) Multiome | 1072 | |
| chr12:103,956,327–103,957,900 | 107.2 kb | Distal (>10kb) Multiome | 1090 | |
| chr12:103,965,409–103,966,472 | 98.6 kb | Distal (>10kb) Multiome | 965 | |
| chr12:104,049,717–104,050,283 | 14.4 kb | Distal (>10kb) Multiome | 295 | |
| chr12:104,056,123–104,056,488 | 8.1 kb | Proximal (<10kb) | 93 | |
| chr12:104,057,791–104,058,196 | 6.3 kb | Proximal (<10kb) | 134 | |
| chr12:104,063,714–104,065,209 | 20 bp | At TSS Multiome | 950 | |
| chr12:104,137,119–104,139,117 | 73.7 kb | Distal (>10kb) Multiome | 796 | |
| chr12:104,154,657–104,155,425 | 90.5 kb | Distal (>10kb) Multiome | 127 | |
| chr12:104,215,473–104,216,381 | 151.3 kb | Distal (>10kb) Multiome | 514 | |
| chr12:104,286,560–104,287,505 | 222.4 kb | Distal (>10kb) Multiome | 878 | |
| chr12:104,288,643–104,289,175 | 224.3 kb | Distal (>10kb) Multiome | 863 | |
| chr12:104,303,321–104,304,194 | 239.2 kb | Distal (>10kb) Multiome | 316 | |
| chr12:105,107,164–105,108,312 | 1043.2 kb | Distal (>10kb) Multiome HiCAR | 1033 |
Genomic view of the HCFC2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.