The protein encoded by this gene is involved in the synthesis of the unbranched glycosaminoglycan hyaluronan, or hyaluronic acid, which is a major constituent of the extracellular matrix. This gene is a member of the NODC/HAS gene family. Compared to the proteins encoded by other members of this gene family, this protein appears to be more of a regulator of hyaluronan synthesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]
Transcription factors with Perturb-seq knockdown data for HAS3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = HAS3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of HAS3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr16:68,843,238–68,844,359 | 261.9 kb | Distal (>10kb) Multiome | 918 | |
| chr16:69,071,809–69,072,879 | 33.2 kb | Distal (>10kb) Multiome | 386 | |
| chr16:69,102,347–69,102,609 | 3.0 kb | Proximal (<10kb) | 13 | |
| chr16:69,105,371–69,107,181 | 599 bp | At TSS Multiome | 631 | |
| chr16:69,107,305–69,107,920 | 1.7 kb | Proximal (<10kb) | 173 | |
| chr16:69,112,744–69,113,134 | 7.1 kb | Proximal (<10kb) | 228 | |
| chr16:69,131,353–69,133,059 | 26.8 kb | Distal (>10kb) Multiome | 961 | |
| chr16:69,186,569–69,187,845 | 81.5 kb | Distal (>10kb) Multiome | 878 | |
| chr16:69,310,637–69,312,153 | 205.6 kb | Distal (>10kb) Multiome | 963 | |
| chr16:69,329,919–69,331,310 | 225.0 kb | Distal (>10kb) Multiome | 977 | |
| chr16:69,339,182–69,340,174 | 234.0 kb | Distal (>10kb) Multiome | 1014 | |
| chr16:69,385,555–69,386,170 | 280.3 kb | Distal (>10kb) Multiome | 857 |
Genomic view of the HAS3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.