Transcription factors with Perturb-seq knockdown data for HAS2-AS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = HAS2-AS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of HAS2-AS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr8:121,537,980–121,538,930 | 101.4 kb | Distal (>10kb) Multiome | 126 | |
| chr8:121,638,932–121,640,661 | 476 bp | At TSS Multiome | 560 | |
| chr8:121,641,223–121,643,313 | 1.7 kb | Proximal (<10kb) Multiome | 595 | |
| chr8:121,649,623–121,650,787 | 10.1 kb | Distal (>10kb) Multiome | 168 | |
| chr8:121,669,074–121,670,012 | 29.7 kb | Distal (>10kb) Multiome | 271 | |
| chr8:121,741,843–121,742,567 | 102.2 kb | Distal (>10kb) Multiome | 238 | |
| chr8:121,756,389–121,757,302 | 116.9 kb | Distal (>10kb) Multiome | 82 | |
| chr8:121,818,237–121,819,660 | 179.5 kb | Distal (>10kb) Multiome | 237 | |
| chr8:121,821,066–121,822,156 | 181.7 kb | Distal (>10kb) Multiome HiCAR | 214 | |
| chr8:122,079,024–122,080,313 | 440.0 kb | Distal (>10kb) Multiome HiCAR | 205 | |
| chr8:122,638,668–122,639,514 | 999.1 kb | Distal (>10kb) Multiome HiCAR | 424 |
Genomic view of the HAS2-AS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.