Transcription factors with Perturb-seq knockdown data for HAGLR. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = HAGLR upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of HAGLR, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:176,171,283–176,172,596 | 5.5 kb | Proximal (<10kb) | 245 | |
| chr2:176,174,544–176,174,869 | 3.3 kb | Proximal (<10kb) | 167 | |
| chr2:176,174,987–176,175,675 | 2.5 kb | Proximal (<10kb) | 273 | |
| chr2:176,177,721–176,178,875 | at TSS | At TSS | 271 | |
| chr2:176,188,129–176,189,887 | 10.0 kb | Proximal (<10kb) | 652 | |
| chr2:176,192,304–176,192,888 | 3.7 kb | Proximal (<10kb) | 56 |
Genomic view of the HAGLR locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.