H4C8
H4 clustered histone 8 | H4/h, H4FH, HIST1H4H

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]

Biological processes 31 terms
DNA binding (GO:0003677)DNA binding (GO:0003677)RNA binding (GO:0003723)chromatin organization (GO:0006325)chromosome (GO:0005694)chromosome, telomeric region (GO:0000781)extracellular exosome (GO:0070062)extracellular exosome (GO:0070062)extracellular region (GO:0005576)membrane (GO:0016020)negative regulation of megakaryocyte differentiation (GO:0045653)nucleoplasm (GO:0005654)nucleoplasm (GO:0005654)nucleoplasm (GO:0005654)nucleosome (GO:0000786)nucleosome (GO:0000786)nucleosome (GO:0000786)nucleosome assembly (GO:0006334)nucleosome assembly (GO:0006334)nucleus (GO:0005634)nucleus (GO:0005634)nucleus (GO:0005634)nucleus (GO:0005634)protein binding (GO:0005515)protein heterodimerization activity (GO:0046982)protein localization to CENP-A containing chromatin (GO:0061644)protein-containing complex (GO:0032991)structural constituent of chromatin (GO:0030527)structural constituent of chromatin (GO:0030527)structural constituent of chromatin (GO:0030527)telomere organization (GO:0032200)
Expression (TPM)
H4C8 — as a Regulated Gene

TFs regulating H4C8 0 TFs

Transcription factors with Perturb-seq knockdown data for H4C8. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = H4C8 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

Data: Effect:
TF Mean coef Binding Outlier TF→Gene link

Elements linked to H4C8

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of H4C8, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility Element Dist. to TSS Link type TFs
chr6:25,992,391–25,993,479 293.0 kb Distal (>10kb) Multiome 1012
chr6:26,020,104–26,022,401 265.3 kb Distal (>10kb) Multiome 920
chr6:26,025,866–26,027,859 259.2 kb Distal (>10kb) Multiome 1194
chr6:26,031,341–26,032,990 253.5 kb Distal (>10kb) Multiome 1083
chr6:26,033,113–26,034,309 251.9 kb Distal (>10kb) Multiome 800
chr6:26,054,926–26,057,287 229.1 kb Distal (>10kb) Multiome 1174
chr6:26,087,383–26,088,017 198.0 kb Distal (>10kb) Multiome 572
chr6:26,103,452–26,105,109 181.7 kb Distal (>10kb) Multiome 1169
chr6:26,122,481–26,124,909 162.8 kb Distal (>10kb) Multiome 1216
chr6:26,156,184–26,158,744 127.6 kb Distal (>10kb) Multiome 1310
chr6:26,171,798–26,172,499 113.6 kb Distal (>10kb) Multiome 580
chr6:26,183,128–26,184,377 102.0 kb Distal (>10kb) Multiome 311
chr6:26,187,874–26,189,538 96.5 kb Distal (>10kb) Multiome 1053
chr6:26,196,695–26,197,676 88.4 kb Distal (>10kb) Multiome 842
chr6:26,198,203–26,200,197 86.2 kb Distal (>10kb) Multiome 939
chr6:26,203,791–26,205,119 81.1 kb Distal (>10kb) Multiome 1071
chr6:26,214,872–26,218,468 68.8 kb Distal (>10kb) Multiome 1096
chr6:26,223,716–26,224,480 61.6 kb Distal (>10kb) Multiome 507
chr6:26,224,856–26,226,566 60.4 kb Distal (>10kb) Multiome 451
chr6:26,239,944–26,240,920 45.3 kb Distal (>10kb) Multiome 375
chr6:26,249,682–26,251,138 35.3 kb Distal (>10kb) Multiome 731
chr6:26,251,339–26,252,079 33.9 kb Distal (>10kb) Multiome 445
chr6:26,270,815–26,273,967 12.7 kb Distal (>10kb) Multiome 742
chr6:26,284,622–26,284,829 808 bp At TSS 271
chr6:26,285,011–26,286,121 50 bp At TSS Multiome 1121
chr6:26,286,225–26,286,630 587 bp At TSS 605
chr6:26,304,972–26,305,585 19.8 kb Distal (>10kb) Multiome 586
chr6:26,312,505–26,313,475 27.3 kb Distal (>10kb) Multiome 896
chr6:26,322,092–26,323,232 37.0 kb Distal (>10kb) Multiome 811
chr6:26,327,476–26,328,318 42.5 kb Distal (>10kb) Multiome 716
chr6:26,341,545–26,342,139 56.2 kb Distal (>10kb) Multiome 716
chr6:26,365,073–26,365,647 79.7 kb Distal (>10kb) Multiome 587
chr6:26,382,957–26,383,951 97.6 kb Distal (>10kb) Multiome 448
chr6:26,421,258–26,421,866 135.8 kb Distal (>10kb) Multiome 473
chr6:26,457,390–26,458,395 172.2 kb Distal (>10kb) Multiome 525
chr6:26,473,842–26,474,357 188.5 kb Distal (>10kb) Multiome 487
chr6:26,520,324–26,522,398 236.3 kb Distal (>10kb) Multiome 1082
chr6:26,532,765–26,533,409 247.4 kb Distal (>10kb) Multiome 830
chr6:26,537,180–26,538,912 251.9 kb Distal (>10kb) Multiome 1114
chr6:26,550,296–26,550,971 265.1 kb Distal (>10kb) Multiome 695
chr6:26,553,165–26,553,642 268.0 kb Distal (>10kb) Multiome 561
chr6:26,556,432–26,557,038 270.9 kb Distal (>10kb) Multiome 676
chr6:26,571,613–26,572,233 286.3 kb Distal (>10kb) Multiome 861
chr6:27,132,200–27,134,276 847.3 kb Distal (>10kb) Multiome HiCAR 975
chr6:27,139,091–27,140,128 853.7 kb Distal (>10kb) Multiome HiCAR 662
chr6:27,145,480–27,148,111 861.4 kb Distal (>10kb) Multiome HiCAR 951

Genome Browser

Genomic view of the H4C8 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.

chr6:25,982,391 – 27,158,111
Proximal 1 kb Distal 10 kb Multiome HiCAR ATAC-seq RNA-seq
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