This gene encodes a transmembrane protein that functions as a receptor for endogenous peptides guanylin and uroguanylin, and the heat-stable E. coli enterotoxin. The encoded protein activates the cystic fibrosis transmembrane conductance regulator. Mutations in this gene are associated with familial diarrhea (autosomal dominant) and meconium ileus (autosomal recessive). [provided by RefSeq, Nov 2016]
Transcription factors with Perturb-seq knockdown data for GUCY2C. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = GUCY2C upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of GUCY2C, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:14,567,263–14,568,383 | 128.8 kb | Distal (>10kb) Multiome | 366 | |
| chr12:14,696,325–14,696,732 | at TSS | At TSS | 55 | |
| chr12:14,698,663–14,698,905 | 2.1 kb | Proximal (<10kb) | 15 | |
| chr12:14,700,039–14,700,272 | 3.5 kb | Proximal (<10kb) | 10 | |
| chr12:14,705,739–14,705,896 | 9.2 kb | Proximal (<10kb) | 3 | |
| chr12:14,710,018–14,711,213 | 14.1 kb | Distal (>10kb) Multiome | 148 | |
| chr12:14,768,991–14,772,177 | 73.3 kb | Distal (>10kb) Multiome | 1151 | |
| chr12:14,773,514–14,775,433 | 77.7 kb | Distal (>10kb) Multiome | 550 | |
| chr12:14,802,974–14,804,269 | 107.0 kb | Distal (>10kb) Multiome | 702 |
Genomic view of the GUCY2C locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.