This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for GTF2B. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = GTF2B upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of GTF2B, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:88,662,071–88,663,175 | 229.0 kb | Distal (>10kb) Multiome | 148 | |
| chr1:88,683,036–88,685,546 | 207.3 kb | Distal (>10kb) Multiome | 961 | |
| chr1:88,890,883–88,892,134 | 47 bp | At TSS Multiome | 949 | |
| chr1:88,892,519–88,892,817 | 874 bp | At TSS | 171 | |
| chr1:88,991,977–88,993,909 | 101.4 kb | Distal (>10kb) Multiome | 925 |
Genomic view of the GTF2B locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.