Transcription factors with Perturb-seq knockdown data for GSCAR. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = GSCAR upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of GSCAR, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:88,683,414–88,684,052 | 9.9 kb | Proximal (<10kb) | 254 | |
| chr5:88,684,266–88,685,701 | 8.3 kb | Proximal (<10kb) | 236 | |
| chr5:88,689,005–88,690,010 | 4.0 kb | Proximal (<10kb) | 213 | |
| chr5:88,690,452–88,691,491 | 2.5 kb | Proximal (<10kb) | 454 | |
| chr5:88,691,943–88,692,893 | 1.1 kb | Proximal (<10kb) | 100 | |
| chr5:88,693,118–88,694,344 | at TSS | At TSS | 143 | |
| chr5:88,701,827–88,702,012 | 7.9 kb | Proximal (<10kb) | 7 | |
| chr5:88,702,421–88,702,679 | 8.4 kb | Proximal (<10kb) | 55 |
Genomic view of the GSCAR locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.