Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling pathways. G proteins are composed of 3 units: alpha, beta and gamma. This gene encodes an alpha subunit and belongs to the G-alpha family. Mutation in this gene, resulting in a gly40-to-arg substitution, is associated with auriculocondylar syndrome, and shown to affect downstream targets in the G protein-coupled endothelin receptor pathway. [provided by RefSeq, Jun 2012]
Transcription factors with Perturb-seq knockdown data for GNAI3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = GNAI3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of GNAI3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:109,249,055–109,250,364 | 299.1 kb | Distal (>10kb) Multiome | 527 | |
| chr1:109,263,250–109,264,042 | 284.9 kb | Distal (>10kb) Multiome | 767 | |
| chr1:109,282,693–109,283,858 | 265.4 kb | Distal (>10kb) Multiome | 850 | |
| chr1:109,306,995–109,308,024 | 241.0 kb | Distal (>10kb) Multiome | 425 | |
| chr1:109,397,369–109,398,671 | 150.6 kb | Distal (>10kb) Multiome | 654 | |
| chr1:109,425,842–109,427,054 | 122.1 kb | Distal (>10kb) Multiome | 922 | |
| chr1:109,466,294–109,467,385 | 82.0 kb | Distal (>10kb) Multiome | 452 | |
| chr1:109,483,147–109,484,806 | 64.7 kb | Distal (>10kb) Multiome | 1031 | |
| chr1:109,493,695–109,494,597 | 54.5 kb | Distal (>10kb) Multiome | 842 | |
| chr1:109,498,180–109,498,608 | 50.2 kb | Distal (>10kb) Multiome | 178 | |
| chr1:109,509,196–109,510,011 | 38.9 kb | Distal (>10kb) Multiome | 448 | |
| chr1:109,532,157–109,533,159 | 15.9 kb | Distal (>10kb) Multiome | 517 | |
| chr1:109,546,363–109,546,566 | 2.0 kb | Proximal (<10kb) | 379 | |
| chr1:109,548,385–109,549,096 | 11 bp | At TSS Multiome | 855 | |
| chr1:109,619,434–109,621,357 | 72.4 kb | Distal (>10kb) Multiome HiCAR | 814 | |
| chr1:109,643,342–109,643,856 | 94.9 kb | Distal (>10kb) Multiome HiCAR | 470 | |
| chr1:109,655,698–109,656,566 | 107.5 kb | Distal (>10kb) Multiome | 707 | |
| chr1:109,667,812–109,668,362 | 119.4 kb | Distal (>10kb) Multiome | 287 | |
| chr1:109,739,695–109,740,873 | 191.6 kb | Distal (>10kb) Multiome | 633 | |
| chr1:109,984,134–109,985,352 | 436.1 kb | Distal (>10kb) Multiome HiCAR | 946 |
Genomic view of the GNAI3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.