This gene encodes a member of the glycosyl hydrolase 35 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature lysosomal enzyme. This enzyme catalyzes the hydrolysis of a terminal beta-linked galactose residue from ganglioside substrates and other glycoconjugates. Mutations in this gene may result in GM1-gangliosidosis and Morquio B syndrome. [provided by RefSeq, Nov 2015]
Transcription factors with Perturb-seq knockdown data for GLB1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = GLB1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of GLB1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:32,815,741–32,817,565 | 280.6 kb | Distal (>10kb) Multiome | 713 | |
| chr3:32,842,816–32,844,607 | 253.6 kb | Distal (>10kb) Multiome | 404 | |
| chr3:32,869,123–32,870,147 | 227.3 kb | Distal (>10kb) Multiome | 264 | |
| chr3:32,902,431–32,903,435 | 194.0 kb | Distal (>10kb) Multiome | 296 | |
| chr3:32,998,701–32,999,462 | 98.1 kb | Distal (>10kb) Multiome | 41 | |
| chr3:33,061,228–33,062,289 | 35.3 kb | Distal (>10kb) Multiome | 238 | |
| chr3:33,096,220–33,097,726 | 79 bp | At TSS Multiome | 905 | |
| chr3:33,113,579–33,114,881 | 17.3 kb | Distal (>10kb) Multiome | 870 | |
| chr3:33,218,068–33,219,328 | 121.7 kb | Distal (>10kb) Multiome | 329 | |
| chr3:33,277,037–33,277,887 | 180.3 kb | Distal (>10kb) Multiome | 534 |
Genomic view of the GLB1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.