Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]
Transcription factors with Perturb-seq knockdown data for GCLM. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = GCLM upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of GCLM, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:93,622,085–93,622,775 | 287.1 kb | Distal (>10kb) Multiome | 282 | |
| chr1:93,678,776–93,679,511 | 230.2 kb | Distal (>10kb) Multiome | 96 | |
| chr1:93,680,733–93,682,277 | 227.5 kb | Distal (>10kb) Multiome | 717 | |
| chr1:93,814,055–93,816,005 | 94.2 kb | Distal (>10kb) Multiome | 533 | |
| chr1:93,820,560–93,821,453 | 88.4 kb | Distal (>10kb) Multiome | 160 | |
| chr1:93,845,985–93,846,892 | 63.2 kb | Distal (>10kb) Multiome | 732 | |
| chr1:93,846,995–93,848,315 | 61.9 kb | Distal (>10kb) Multiome | 954 | |
| chr1:93,878,726–93,879,400 | 30.3 kb | Distal (>10kb) Multiome | 851 | |
| chr1:93,908,647–93,910,233 | 160 bp | At TSS Multiome | 901 | |
| chr1:94,045,555–94,046,076 | 136.4 kb | Distal (>10kb) Multiome | 556 | |
| chr1:94,148,998–94,149,784 | 239.9 kb | Distal (>10kb) Multiome | 44 |
Genomic view of the GCLM locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.