The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. This gene encodes the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The encoded enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 14. [provided by RefSeq, Jan 2010]
Transcription factors with Perturb-seq knockdown data for GCAT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = GCAT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of GCAT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr22:37,518,831–37,519,884 | 288.4 kb | Distal (>10kb) Multiome | 548 | |
| chr22:37,545,569–37,546,885 | 261.6 kb | Distal (>10kb) Multiome | 682 | |
| chr22:37,559,893–37,560,965 | 247.6 kb | Distal (>10kb) Multiome | 589 | |
| chr22:37,569,013–37,570,549 | 238.4 kb | Distal (>10kb) Multiome | 695 | |
| chr22:37,608,044–37,609,455 | 199.2 kb | Distal (>10kb) Multiome | 920 | |
| chr22:37,639,157–37,639,951 | 168.4 kb | Distal (>10kb) Multiome | 427 | |
| chr22:37,658,172–37,659,508 | 149.4 kb | Distal (>10kb) Multiome | 722 | |
| chr22:37,674,916–37,675,641 | 132.5 kb | Distal (>10kb) Multiome | 607 | |
| chr22:37,676,590–37,677,674 | 130.7 kb | Distal (>10kb) Multiome | 655 | |
| chr22:37,686,104–37,686,784 | 121.5 kb | Distal (>10kb) Multiome | 866 | |
| chr22:37,696,496–37,697,685 | 111.0 kb | Distal (>10kb) Multiome | 428 | |
| chr22:37,745,641–37,746,995 | 61.8 kb | Distal (>10kb) Multiome | 627 | |
| chr22:37,802,124–37,802,989 | 5.5 kb | Proximal (<10kb) Multiome | 603 | |
| chr22:37,803,341–37,803,517 | 4.4 kb | Proximal (<10kb) | 376 | |
| chr22:37,804,679–37,805,768 | 2.7 kb | Proximal (<10kb) Multiome | 763 | |
| chr22:37,807,436–37,808,267 | 108 bp | At TSS Multiome | 766 | |
| chr22:37,817,672–37,819,114 | 9.9 kb | Proximal (<10kb) Multiome | 713 | |
| chr22:37,824,524–37,825,593 | 17.0 kb | Distal (>10kb) Multiome | 173 | |
| chr22:37,843,748–37,844,743 | 36.5 kb | Distal (>10kb) Multiome | 798 | |
| chr22:37,849,215–37,849,709 | 41.5 kb | Distal (>10kb) Multiome | 753 | |
| chr22:37,905,849–37,906,846 | 98.3 kb | Distal (>10kb) Multiome | 552 | |
| chr22:37,933,777–37,934,256 | 126.2 kb | Distal (>10kb) Multiome | 679 | |
| chr22:37,953,153–37,954,120 | 145.7 kb | Distal (>10kb) Multiome | 617 | |
| chr22:37,983,021–37,985,110 | 176.5 kb | Distal (>10kb) Multiome | 228 | |
| chr22:38,052,312–38,052,803 | 244.6 kb | Distal (>10kb) Multiome | 529 | |
| chr22:38,057,008–38,057,702 | 249.4 kb | Distal (>10kb) Multiome | 656 | |
| chr22:38,080,912–38,081,618 | 273.2 kb | Distal (>10kb) Multiome | 175 | |
| chr22:38,097,697–38,098,236 | 290.0 kb | Distal (>10kb) Multiome | 186 |
Genomic view of the GCAT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.