Transcription factors with Perturb-seq knockdown data for GAU1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = GAU1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of GAU1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:4,538,204–4,539,165 | 181.4 kb | Distal (>10kb) Multiome | 740 | |
| chr12:4,562,041–4,562,555 | 157.8 kb | Distal (>10kb) Multiome | 486 | |
| chr12:4,604,713–4,605,166 | 115.2 kb | Distal (>10kb) Multiome | 580 | |
| chr12:4,648,527–4,649,423 | 71.1 kb | Distal (>10kb) Multiome | 645 | |
| chr12:4,720,395–4,720,946 | 629 bp | At TSS Multiome | 69 | |
| chr12:4,809,137–4,810,290 | 89.3 kb | Distal (>10kb) Multiome | 246 |
Genomic view of the GAU1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.