GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. Transcript variants utilizing three different alternative non-coding first exons have been described. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for GABRA5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = GABRA5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of GABRA5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr15:26,628,220–26,629,757 | 237.8 kb | Distal (>10kb) Multiome | 360 | |
| chr15:26,767,146–26,767,831 | 99.6 kb | Distal (>10kb) Multiome | 143 | |
| chr15:26,771,278–26,773,906 | 93.8 kb | Distal (>10kb) Multiome | 353 | |
| chr15:26,864,801–26,864,991 | 2.1 kb | Proximal (<10kb) | 106 | |
| chr15:26,866,706–26,868,257 | 34 bp | At TSS Multiome | 172 | |
| chr15:26,970,760–26,971,806 | 104.1 kb | Distal (>10kb) Multiome | 183 |
Genomic view of the GABRA5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.