Transcription factors with Perturb-seq knockdown data for FRMD6-AS2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = FRMD6-AS2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of FRMD6-AS2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr14:51,650,395–51,650,572 | 1.2 kb | Proximal (<10kb) | 13 | |
| chr14:51,650,673–51,652,859 | 10 bp | At TSS Multiome | 783 | |
| chr14:51,776,047–51,776,802 | 124.7 kb | Distal (>10kb) Multiome | 249 | |
| chr14:51,826,826–51,827,456 | 175.2 kb | Distal (>10kb) Multiome | 39 | |
| chr14:51,827,934–51,828,961 | 176.7 kb | Distal (>10kb) Multiome | 229 | |
| chr14:51,846,633–51,847,447 | 195.3 kb | Distal (>10kb) Multiome | 292 |
Genomic view of the FRMD6-AS2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.