This gene encodes the folylpolyglutamate synthetase enzyme. This enzyme has a central role in establishing and maintaining both cytosolic and mitochondrial folylpolyglutamate concentrations and, therefore, is essential for folate homeostasis and the survival of proliferating cells. This enzyme catalyzes the ATP-dependent addition of glutamate moieties to folate and folate derivatives. Alternative splicing results in transcript variants encoding different isoforms. [provided by RefSeq, Jan 2014]
Transcription factors with Perturb-seq knockdown data for FPGS. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = FPGS upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of FPGS, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr9:127,567,936–127,569,972 | 233.7 kb | Distal (>10kb) Multiome | 660 | |
| chr9:127,608,129–127,608,913 | 194.4 kb | Distal (>10kb) Multiome | 700 | |
| chr9:127,611,734–127,612,761 | 190.7 kb | Distal (>10kb) Multiome | 566 | |
| chr9:127,699,155–127,699,632 | 103.4 kb | Distal (>10kb) Multiome | 440 | |
| chr9:127,715,013–127,716,345 | 87.1 kb | Distal (>10kb) Multiome | 735 | |
| chr9:127,734,444–127,735,606 | 67.6 kb | Distal (>10kb) Multiome | 836 | |
| chr9:127,741,739–127,743,092 | 60.1 kb | Distal (>10kb) Multiome | 367 | |
| chr9:127,754,123–127,755,506 | 47.5 kb | Distal (>10kb) Multiome | 279 | |
| chr9:127,770,807–127,771,925 | 31.4 kb | Distal (>10kb) Multiome | 550 | |
| chr9:127,784,682–127,787,009 | 16.3 kb | Distal (>10kb) Multiome | 858 | |
| chr9:127,802,385–127,803,632 | 45 bp | At TSS Multiome | 867 | |
| chr9:127,809,338–127,810,272 | 7.0 kb | Proximal (<10kb) Multiome | 276 | |
| chr9:127,825,222–127,826,046 | 22.7 kb | Distal (>10kb) Multiome | 451 | |
| chr9:127,828,234–127,829,024 | 25.7 kb | Distal (>10kb) Multiome | 521 | |
| chr9:127,877,282–127,878,202 | 74.9 kb | Distal (>10kb) Multiome | 531 | |
| chr9:127,897,316–127,897,796 | 94.6 kb | Distal (>10kb) Multiome | 437 | |
| chr9:127,898,861–127,900,094 | 96.7 kb | Distal (>10kb) Multiome | 630 | |
| chr9:127,916,386–127,917,505 | 114.2 kb | Distal (>10kb) Multiome | 474 | |
| chr9:127,926,756–127,928,574 | 124.6 kb | Distal (>10kb) Multiome HiCAR | 411 | |
| chr9:127,930,321–127,931,068 | 127.9 kb | Distal (>10kb) Multiome | 537 | |
| chr9:127,937,578–127,938,244 | 135.0 kb | Distal (>10kb) Multiome | 674 | |
| chr9:127,955,445–127,955,915 | 152.7 kb | Distal (>10kb) Multiome | 540 | |
| chr9:127,980,172–127,981,966 | 178.2 kb | Distal (>10kb) Multiome | 691 | |
| chr9:128,033,544–128,034,106 | 231.0 kb | Distal (>10kb) Multiome | 69 | |
| chr9:128,034,940–128,035,527 | 232.4 kb | Distal (>10kb) Multiome | 480 | |
| chr9:128,066,577–128,068,774 | 264.5 kb | Distal (>10kb) Multiome | 874 | |
| chr9:128,097,872–128,098,910 | 295.5 kb | Distal (>10kb) Multiome | 712 |
Genomic view of the FPGS locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.