Metabolic N-oxidation of diet-derived amino-trimethylamine (TMA) is mediated by flavin-containing monooxygenase and is subject to an inherited FMO3 polymorphism in man. This results in a small subpopulation with reduced TMA N-oxidation capacity and causes fish odor syndrome (Trimethylaminuria). Three forms of the enzyme are encoded by genes clustered in the 1q23-q25 region. Flavin-containing monooxygenases are NADPH-dependent flavoenzymes that catalyzes the oxidation of soft nucleophilic heteroatom centers in drugs, pesticides, and xenobiotics. [provided by RefSeq, Jan 2015]
Transcription factors with Perturb-seq knockdown data for FMO4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = FMO4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of FMO4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:171,219,608–171,221,098 | 93.4 kb | Distal (>10kb) Multiome | 330 | |
| chr1:171,310,628–171,311,301 | 2.9 kb | Proximal (<10kb) | 14 | |
| chr1:171,313,680–171,314,256 | 115 bp | At TSS Multiome | 512 | |
| chr1:171,396,571–171,398,669 | 82.9 kb | Distal (>10kb) Multiome | 230 | |
| chr1:171,431,769–171,432,338 | 118.0 kb | Distal (>10kb) Multiome | 65 | |
| chr1:171,484,908–171,486,487 | 171.3 kb | Distal (>10kb) Multiome | 978 | |
| chr1:171,508,885–171,509,949 | 195.2 kb | Distal (>10kb) Multiome | 157 |
Genomic view of the FMO4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.