The protein encoded by this gene is a member of a family of proteins containing a four-and-a-half LIM domain, which is a highly conserved double zinc finger motif. The encoded protein has been shown to interact with the cancer developmental regulators SMAD2, SMAD3, and SMAD4, the skeletal muscle myogenesis protein MyoD, and the high-affinity IgE beta chain regulator MZF-1. This protein may be involved in tumor suppression, repression of MyoD expression, and repression of IgE receptor expression. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
Transcription factors with Perturb-seq knockdown data for FHL3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = FHL3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of FHL3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:37,752,766–37,753,277 | 252.5 kb | Distal (>10kb) Multiome | 223 | |
| chr1:37,793,768–37,794,528 | 211.4 kb | Distal (>10kb) Multiome | 567 | |
| chr1:37,807,583–37,809,057 | 197.3 kb | Distal (>10kb) Multiome | 755 | |
| chr1:37,859,081–37,860,381 | 145.8 kb | Distal (>10kb) Multiome | 939 | |
| chr1:37,931,446–37,932,215 | 73.6 kb | Distal (>10kb) Multiome | 559 | |
| chr1:37,946,605–37,947,220 | 58.5 kb | Distal (>10kb) Multiome | 610 | |
| chr1:37,989,644–37,990,549 | 15.4 kb | Distal (>10kb) Multiome | 1133 | |
| chr1:37,995,790–37,996,610 | 9.3 kb | Proximal (<10kb) Multiome | 313 | |
| chr1:38,001,158–38,001,463 | 4.0 kb | Proximal (<10kb) | 149 | |
| chr1:38,004,771–38,006,024 | 78 bp | At TSS Multiome | 442 | |
| chr1:38,009,077–38,009,551 | 3.6 kb | Proximal (<10kb) | 354 | |
| chr1:38,012,220–38,013,284 | 7.1 kb | Proximal (<10kb) Multiome | 1074 | |
| chr1:38,030,338–38,030,974 | 25.0 kb | Distal (>10kb) Multiome | 225 | |
| chr1:38,045,344–38,048,035 | 41.5 kb | Distal (>10kb) Multiome | 542 | |
| chr1:38,803,885–38,804,528 | 798.8 kb | Distal (>10kb) Multiome HiCAR | 257 | |
| chr1:38,817,720–38,818,696 | 812.8 kb | Distal (>10kb) Multiome HiCAR | 375 | |
| chr1:38,859,087–38,860,584 | 854.3 kb | Distal (>10kb) Multiome HiCAR | 848 |
Genomic view of the FHL3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.