This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is similar to xenopus early mitotic inhibitor-1 (Emi1), which is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Dec 2008]
Transcription factors with Perturb-seq knockdown data for FBXO5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = FBXO5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of FBXO5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:151,589,007–151,590,183 | 1393.8 kb | Distal (>10kb) Multiome HiCAR | 147 | |
| chr6:152,681,820–152,683,597 | 300.4 kb | Distal (>10kb) Multiome | 260 | |
| chr6:152,976,675–152,976,927 | 6.1 kb | Proximal (<10kb) | 37 | |
| chr6:152,982,060–152,984,380 | 255 bp | At TSS Multiome | 1096 | |
| chr6:152,993,560–152,993,723 | 10.0 kb | Proximal (<10kb) | 25 | |
| chr6:153,001,940–153,003,776 | 19.3 kb | Distal (>10kb) Multiome | 953 | |
| chr6:153,129,787–153,132,406 | 147.8 kb | Distal (>10kb) Multiome | 562 |
Genomic view of the FBXO5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.