Transcription factors with Perturb-seq knockdown data for FAM78B. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = FAM78B upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of FAM78B, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:166,158,717–166,159,201 | 7.8 kb | Proximal (<10kb) | 12 | |
| chr1:166,159,484–166,160,279 | 6.7 kb | Proximal (<10kb) | 93 | |
| chr1:166,164,826–166,167,581 | 73 bp | At TSS Multiome | 578 | |
| chr1:166,206,285–166,207,407 | 39.9 kb | Distal (>10kb) Multiome | 105 | |
| chr1:166,303,740–166,306,077 | 137.4 kb | Distal (>10kb) Multiome HiCAR | 118 | |
| chr1:166,409,171–166,410,386 | 242.7 kb | Distal (>10kb) Multiome HiCAR | 248 |
Genomic view of the FAM78B locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.