Transcription factors with Perturb-seq knockdown data for FAM217A. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = FAM217A upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of FAM217A, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:3,848,709–3,849,895 | 229.9 kb | Distal (>10kb) Multiome | 495 | |
| chr6:4,018,202–4,019,132 | 60.4 kb | Distal (>10kb) Multiome | 1077 | |
| chr6:4,020,917–4,022,040 | 57.7 kb | Distal (>10kb) Multiome | 909 | |
| chr6:4,078,571–4,079,606 | 10 bp | At TSS Multiome | 164 | |
| chr6:4,134,917–4,136,384 | 56.7 kb | Distal (>10kb) Multiome | 976 |
Genomic view of the FAM217A locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.