This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator and have a role during development. It can act as a mediator of chemoresistance and cell survival in Ewing sarcoma cells, where this gene is up-regulated via a micro-RNA that binds to the 3' UTR of the transcript. A similar protein in mice acts as a transcriptional activator. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2013]
Transcription factors with Perturb-seq knockdown data for EYA3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = EYA3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of EYA3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:27,830,416–27,831,407 | 257.8 kb | Distal (>10kb) Multiome | 899 | |
| chr1:27,872,169–27,873,143 | 216.1 kb | Distal (>10kb) Multiome | 412 | |
| chr1:27,914,296–27,915,026 | 173.9 kb | Distal (>10kb) Multiome | 866 | |
| chr1:27,934,817–27,935,435 | 153.6 kb | Distal (>10kb) Multiome | 308 | |
| chr1:27,959,376–27,960,547 | 128.8 kb | Distal (>10kb) Multiome | 711 | |
| chr1:28,088,113–28,088,897 | 96 bp | At TSS Multiome | 827 | |
| chr1:28,104,959–28,105,483 | 16.7 kb | Distal (>10kb) Multiome | 240 | |
| chr1:28,232,593–28,233,160 | 144.4 kb | Distal (>10kb) Multiome | 666 | |
| chr1:28,235,692–28,236,588 | 147.5 kb | Distal (>10kb) Multiome | 862 | |
| chr1:28,247,126–28,247,685 | 158.7 kb | Distal (>10kb) Multiome | 544 | |
| chr1:28,258,840–28,260,822 | 171.8 kb | Distal (>10kb) Multiome | 910 | |
| chr1:28,369,387–28,370,499 | 281.2 kb | Distal (>10kb) Multiome | 961 |
Genomic view of the EYA3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.