ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for ETFA. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ETFA upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ETFA, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr15:76,033,743–76,034,907 | 277.2 kb | Distal (>10kb) Multiome | 131 | |
| chr15:76,058,953–76,060,346 | 251.6 kb | Distal (>10kb) Multiome | 463 | |
| chr15:76,139,361–76,140,331 | 171.7 kb | Distal (>10kb) Multiome | 257 | |
| chr15:76,253,843–76,254,335 | 57.4 kb | Distal (>10kb) Multiome | 59 | |
| chr15:76,310,960–76,312,031 | 30 bp | At TSS Multiome | 980 | |
| chr15:76,335,834–76,337,186 | 25.2 kb | Distal (>10kb) Multiome | 781 | |
| chr15:76,337,664–76,338,596 | 26.7 kb | Distal (>10kb) Multiome | 603 | |
| chr15:76,341,048–76,343,196 | 31.2 kb | Distal (>10kb) Multiome | 257 | |
| chr15:76,345,613–76,346,889 | 34.9 kb | Distal (>10kb) Multiome | 577 |
Genomic view of the ETFA locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.