This gene encodes the transmembrane protein kinase inositol-requiring enzyme 1. The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1. [provided by RefSeq, Aug 2017]
Transcription factors with Perturb-seq knockdown data for ERN1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ERN1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ERN1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:63,841,547–63,843,442 | 287.2 kb | Distal (>10kb) Multiome | 1203 | |
| chr17:63,848,836–63,849,566 | 281.0 kb | Distal (>10kb) Multiome | 658 | |
| chr17:63,960,119–63,962,604 | 168.2 kb | Distal (>10kb) Multiome | 445 | |
| chr17:63,998,051–63,998,826 | 131.8 kb | Distal (>10kb) Multiome | 408 | |
| chr17:64,129,318–64,130,921 | 96 bp | At TSS Multiome | 978 | |
| chr17:64,145,473–64,147,600 | 15.8 kb | Distal (>10kb) Multiome | 1196 | |
| chr17:64,262,431–64,263,974 | 133.2 kb | Distal (>10kb) Multiome | 914 |
Genomic view of the ERN1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.