This gene encodes a member of a family of transmembrane proteins that function as receptors for ephrin-B family proteins. Unlike other members of this family, the encoded protein does not contain a functional kinase domain. Activity of this protein can influence cell adhesion and migration. Expression of this gene is downregulated during tumor progression, suggesting that the protein may suppress tumor invasion and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Transcription factors with Perturb-seq knockdown data for EPHB6. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = EPHB6 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of EPHB6, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr7:142,696,147–142,697,139 | 158.2 kb | Distal (>10kb) Multiome | 120 | |
| chr7:142,748,805–142,749,672 | 105.8 kb | Distal (>10kb) Multiome | 74 | |
| chr7:142,770,332–142,771,391 | 84.2 kb | Distal (>10kb) Multiome | 150 | |
| chr7:142,782,876–142,783,641 | 71.8 kb | Distal (>10kb) Multiome | 448 | |
| chr7:142,813,144–142,813,828 | 41.6 kb | Distal (>10kb) Multiome | 250 | |
| chr7:142,853,711–142,856,176 | 105 bp | At TSS Multiome | 583 | |
| chr7:142,885,313–142,886,162 | 30.7 kb | Distal (>10kb) Multiome | 160 | |
| chr7:143,041,996–143,043,331 | 187.8 kb | Distal (>10kb) Multiome | 258 | |
| chr7:143,284,394–143,285,772 | 429.9 kb | Distal (>10kb) Multiome HiCAR | 381 | |
| chr7:143,287,670–143,288,978 | 433.2 kb | Distal (>10kb) Multiome HiCAR | 878 |
Genomic view of the EPHB6 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.