This gene can encode a mitochondrial enzyme that is thought to convert L-fuconate to 2-keto-3-deoxy-L-fuconate. This locus was originally identified as the source of antisense RNAs of the adjacent thymidylate synthase gene. Splice variants at this locus may contain an alternate 3' exon that is complementary to the 3'UTR and terminal intron of the thymidylate synthase (TS) RNA and may downregulate TS expression. [provided by RefSeq, Aug 2017]
Transcription factors with Perturb-seq knockdown data for ENOSF1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ENOSF1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ENOSF1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr18:499,984–501,449 | 185.7 kb | Distal (>10kb) Multiome | 306 | |
| chr18:516,064–516,762 | 170.1 kb | Distal (>10kb) Multiome | 96 | |
| chr18:596,942–597,511 | 89.4 kb | Distal (>10kb) Multiome | 522 | |
| chr18:657,152–658,975 | 28.1 kb | Distal (>10kb) Multiome | 968 | |
| chr18:685,561–685,725 | 721 bp | At TSS | 17 | |
| chr18:685,991–687,114 | 98 bp | At TSS Multiome | 127 | |
| chr18:711,531–712,094 | 567 bp | At TSS | 511 | |
| chr18:712,215–713,458 | 26.3 kb | Distal (>10kb) Multiome | 797 | |
| chr18:811,520–813,366 | 126.0 kb | Distal (>10kb) Multiome HiCAR | 893 | |
| chr18:906,154–908,403 | 220.3 kb | Distal (>10kb) Multiome | 273 | |
| chr18:2,570,743–2,572,078 | 1884.9 kb | Distal (>10kb) Multiome HiCAR | 906 |
Genomic view of the ENOSF1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.