This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme is found in skeletal muscle cells in the adult where it may play a role in muscle development and regeneration. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene have be associated glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Jul 2010]
Transcription factors with Perturb-seq knockdown data for ENO3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ENO3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ENO3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:4,703,669–4,704,637 | 246.9 kb | Distal (>10kb) Multiome | 854 | |
| chr17:4,708,553–4,709,232 | 242.4 kb | Distal (>10kb) Multiome | 71 | |
| chr17:4,709,724–4,711,094 | 240.7 kb | Distal (>10kb) Multiome | 771 | |
| chr17:4,730,794–4,732,115 | 219.5 kb | Distal (>10kb) Multiome | 936 | |
| chr17:4,789,618–4,790,520 | 160.8 kb | Distal (>10kb) Multiome | 816 | |
| chr17:4,795,974–4,796,433 | 155.0 kb | Distal (>10kb) Multiome | 751 | |
| chr17:4,806,693–4,807,619 | 144.0 kb | Distal (>10kb) Multiome | 494 | |
| chr17:4,832,863–4,833,956 | 117.7 kb | Distal (>10kb) Multiome | 730 | |
| chr17:4,898,962–4,900,112 | 51.6 kb | Distal (>10kb) Multiome | 257 | |
| chr17:4,900,249–4,901,057 | 50.3 kb | Distal (>10kb) Multiome | 145 | |
| chr17:4,909,117–4,909,538 | 41.8 kb | Distal (>10kb) Multiome | 553 | |
| chr17:4,939,475–4,941,034 | 10.9 kb | Distal (>10kb) Multiome | 821 | |
| chr17:4,947,426–4,950,636 | 827 bp | At TSS Multiome | 1080 | |
| chr17:4,966,739–4,968,875 | 16.5 kb | Distal (>10kb) Multiome | 982 | |
| chr17:4,986,950–4,987,983 | 36.5 kb | Distal (>10kb) Multiome | 881 | |
| chr17:4,996,838–4,998,964 | 46.6 kb | Distal (>10kb) Multiome | 759 | |
| chr17:5,111,339–5,112,612 | 160.9 kb | Distal (>10kb) Multiome | 864 | |
| chr17:5,191,578–5,192,305 | 240.8 kb | Distal (>10kb) Multiome | 771 |
Genomic view of the ENO3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.