Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for EIF5B. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = EIF5B upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of EIF5B, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:99,081,586–99,082,682 | 255.3 kb | Distal (>10kb) Multiome | 73 | |
| chr2:99,140,631–99,142,044 | 196.0 kb | Distal (>10kb) Multiome | 976 | |
| chr2:99,154,503–99,155,535 | 182.4 kb | Distal (>10kb) Multiome | 1013 | |
| chr2:99,180,725–99,181,615 | 156.3 kb | Distal (>10kb) Multiome | 1014 | |
| chr2:99,335,843–99,338,098 | 69 bp | At TSS Multiome | 1129 | |
| chr2:99,489,057–99,490,837 | 152.8 kb | Distal (>10kb) Multiome | 982 |
Genomic view of the EIF5B locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.