Eukaryotic translation initiation factor-3 (eIF3), the largest of the eIFs, is a multiprotein complex composed of at least ten nonidentical subunits. The complex binds to the 40S ribosome and helps maintain the 40S and 60S ribosomal subunits in a dissociated state. It is also thought to play a role in the formation of the 40S initiation complex by interacting with the ternary complex of eIF2/GTP/methionyl-tRNA, and by promoting mRNA binding. The protein encoded by this gene is the major RNA binding subunit of the eIF3 complex. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for EIF3D. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = EIF3D upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of EIF3D, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr22:36,386,961–36,388,711 | 141.0 kb | Distal (>10kb) Multiome | 830 | |
| chr22:36,409,675–36,410,687 | 119.0 kb | Distal (>10kb) Multiome | 707 | |
| chr22:36,454,782–36,455,724 | 74.0 kb | Distal (>10kb) Multiome | 598 | |
| chr22:36,459,142–36,459,906 | 69.7 kb | Distal (>10kb) Multiome | 269 | |
| chr22:36,481,299–36,482,295 | 47.2 kb | Distal (>10kb) Multiome | 842 | |
| chr22:36,506,747–36,507,706 | 22.1 kb | Distal (>10kb) Multiome | 964 | |
| chr22:36,528,683–36,529,724 | 148 bp | At TSS Multiome | 1012 | |
| chr22:36,775,650–36,776,664 | 247.1 kb | Distal (>10kb) Multiome HiCAR | 472 |
Genomic view of the EIF3D locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.