The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]
Transcription factors with Perturb-seq knockdown data for EIF2S1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = EIF2S1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of EIF2S1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr14:66,345,975–66,346,460 | 1014.2 kb | Distal (>10kb) Multiome HiCAR | 132 | |
| chr14:67,240,431–67,242,037 | 119.0 kb | Distal (>10kb) Multiome | 924 | |
| chr14:67,359,034–67,360,833 | 319 bp | At TSS Multiome | 891 | |
| chr14:67,427,006–67,427,629 | 67.0 kb | Distal (>10kb) Multiome | 288 | |
| chr14:67,514,900–67,515,814 | 155.0 kb | Distal (>10kb) Multiome | 411 | |
| chr14:67,532,784–67,533,987 | 173.0 kb | Distal (>10kb) Multiome | 684 | |
| chr14:67,541,829–67,542,290 | 181.7 kb | Distal (>10kb) Multiome | 187 | |
| chr14:67,599,595–67,600,617 | 239.9 kb | Distal (>10kb) Multiome | 746 | |
| chr14:67,619,336–67,620,756 | 259.5 kb | Distal (>10kb) Multiome | 794 |
Genomic view of the EIF2S1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.