EIF2AK3
eukaryotic translation initiation factor 2 alpha kinase 3 | PEK, PERK

The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015]

Member of: DE-2
Biological processes 71 terms
ATP binding (GO:0005524)ER overload response (GO:0006983)Hsp90 protein binding (GO:0051879)PERK-mediated unfolded protein response (GO:0036499)PERK-mediated unfolded protein response (GO:0036499)angiogenesis (GO:0001525)bone mineralization (GO:0030282)calcium-mediated signaling (GO:0019722)cellular response to amino acid starvation (GO:0034198)cellular response to amino acid starvation (GO:0034198)cellular response to cold (GO:0070417)cellular response to glucose starvation (GO:0042149)chondrocyte development (GO:0002063)cytoplasm (GO:0005737)cytoplasm (GO:0005737)cytosol (GO:0005829)endocrine pancreas development (GO:0031018)endoplasmic reticulum (GO:0005783)endoplasmic reticulum (GO:0005783)endoplasmic reticulum (GO:0005783)endoplasmic reticulum (GO:0005783)endoplasmic reticulum membrane (GO:0005789)endoplasmic reticulum membrane (GO:0005789)endoplasmic reticulum membrane (GO:0005789)endoplasmic reticulum membrane (GO:0005789)endoplasmic reticulum organization (GO:0007029)endoplasmic reticulum unfolded protein response (GO:0030968)endoplasmic reticulum unfolded protein response (GO:0030968)enzyme binding (GO:0019899)eukaryotic translation initiation factor 2alpha kinase activity (GO:0004694)eukaryotic translation initiation factor 2alpha kinase activity (GO:0004694)eukaryotic translation initiation factor 2alpha kinase activity (GO:0004694)eukaryotic translation initiation factor 2alpha kinase activity (GO:0004694)identical protein binding (GO:0042802)insulin-like growth factor receptor signaling pathway (GO:0048009)membrane (GO:0016020)misfolded protein binding (GO:0051787)mitochondria-associated endoplasmic reticulum membrane contact site (GO:0044233)negative regulation of myelination (GO:0031642)negative regulation of translation (GO:0017148)negative regulation of translation (GO:0017148)negative regulation of translation (GO:0017148)negative regulation of translation in response to stress (GO:0032055)negative regulation of translational initiation (GO:0045947)negative regulation of translational initiation in response to stress (GO:0032057)non-membrane spanning protein tyrosine kinase activity (GO:0004715)nucleus (GO:0005634)ossification (GO:0001503)perinuclear region of cytoplasm (GO:0048471)positive regulation of gene expression (GO:0010628)positive regulation of protein localization to nucleus (GO:1900182)positive regulation of transcription by RNA polymerase I (GO:0045943)positive regulation of vascular endothelial growth factor production (GO:0010575)protein binding (GO:0005515)protein kinase activity (GO:0004672)protein kinase activity (GO:0004672)protein kinase activity (GO:0004672)protein phosphatase binding (GO:0019903)protein serine kinase activity (GO:0106310)protein serine/threonine kinase activity (GO:0004674)protein serine/threonine kinase activity (GO:0004674)protein tyrosine kinase activity (GO:0004713)protein tyrosine kinase activity (GO:0004713)regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway (GO:1902235)regulation of translation initiation in response to endoplasmic reticulum stress (GO:0036491)regulation of translational initiation (GO:0006446)response to endoplasmic reticulum stress (GO:0034976)response to endoplasmic reticulum stress (GO:0034976)response to manganese-induced endoplasmic reticulum stress (GO:1990737)skeletal system development (GO:0001501)translation regulator activity (GO:0045182)
Expression (TPM)
EIF2AK3 — as a Regulated Gene

TFs regulating EIF2AK3 0 TFs

Transcription factors with Perturb-seq knockdown data for EIF2AK3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = EIF2AK3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

Data: Effect:
TF Mean coef Binding Outlier TF→Gene link

Elements linked to EIF2AK3

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of EIF2AK3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility Element Dist. to TSS Link type TFs
chr2:88,329,732–88,330,828 361.1 kb Distal (>10kb) Multiome 408
chr2:88,350,256–88,351,496 340.4 kb Distal (>10kb) Multiome 374
chr2:88,415,840–88,416,802 275.2 kb Distal (>10kb) Multiome 135
chr2:88,451,554–88,453,275 238.9 kb Distal (>10kb) Multiome 267
chr2:88,595,791–88,596,888 95.0 kb Distal (>10kb) Multiome 183
chr2:88,626,410–88,628,619 63.8 kb Distal (>10kb) Multiome 1048
chr2:88,691,023–88,692,567 162 bp At TSS Multiome 1023
chr2:88,698,542–88,699,352 7.1 kb Proximal (<10kb) 159
chr2:88,764,838–88,766,065 74.0 kb Distal (>10kb) Multiome 209

Genome Browser

Genomic view of the EIF2AK3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.

chr2:88,319,732 – 88,776,065
Proximal 1 kb Distal 10 kb Multiome HiCAR ATAC-seq RNA-seq