This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for EEF2K. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = EEF2K upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of EEF2K, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr16:21,952,487–21,953,795 | 253.1 kb | Distal (>10kb) Multiome | 1014 | |
| chr16:22,007,549–22,008,682 | 198.3 kb | Distal (>10kb) Multiome | 685 | |
| chr16:22,189,506–22,191,692 | 15.4 kb | Distal (>10kb) Multiome | 989 | |
| chr16:22,195,474–22,196,347 | 10.4 kb | Distal (>10kb) Multiome | 886 | |
| chr16:22,205,924–22,206,847 | 23 bp | At TSS Multiome | 1038 | |
| chr16:22,296,790–22,298,718 | 92.3 kb | Distal (>10kb) Multiome HiCAR | 1288 | |
| chr16:22,373,797–22,375,383 | 168.4 kb | Distal (>10kb) Multiome | 951 | |
| chr16:22,436,881–22,437,622 | 230.8 kb | Distal (>10kb) Multiome | 642 |
Genomic view of the EEF2K locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.