DUOX1
dual oxidase 1 | LNOX1, NOXEF1, THOX1

The protein encoded by this gene is a glycoprotein and a member of the NADPH oxidase family. The synthesis of thyroid hormone is catalyzed by a protein complex located at the apical membrane of thyroid follicular cells. This complex contains an iodide transporter, thyroperoxidase, and a peroxide generating system that includes proteins encoded by this gene and the similar DUOX2 gene. This protein is known as dual oxidase because it has both a peroxidase homology domain and a gp91phox domain. This protein generates hydrogen peroxide and thereby plays a role in the activity of thyroid peroxidase, lactoperoxidase, and in lactoperoxidase-mediated antimicrobial defense at mucosal surfaces. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq, Jul 2012]

Biological processes 58 terms
FAD binding (GO:0071949)FAD binding (GO:0071949)FAD binding (GO:0071949)NAD(P)H oxidase H2O2-forming activity (GO:0016174)NAD(P)H oxidase H2O2-forming activity (GO:0016174)NAD(P)H oxidase H2O2-forming activity (GO:0016174)NAD(P)H oxidase H2O2-forming activity (GO:0016174)NADP binding (GO:0050661)NADPH binding (GO:0070402)NADPH binding (GO:0070402)NADPH binding (GO:0070402)NADPH oxidase complex (GO:0043020)antiviral innate immune response (GO:0140374)antiviral innate immune response (GO:0140374)apical plasma membrane (GO:0016324)apical plasma membrane (GO:0016324)apical plasma membrane (GO:0016324)calcium ion binding (GO:0005509)calcium ion binding (GO:0005509)cell leading edge (GO:0031252)cell leading edge (GO:0031252)cell surface (GO:0009986)cellular response to cytokine stimulus (GO:0071345)cuticle development (GO:0042335)cytokine-mediated signaling pathway (GO:0019221)defense response (GO:0006952)endoplasmic reticulum (GO:0005783)heme binding (GO:0020037)heme binding (GO:0020037)heme binding (GO:0020037)hydrogen peroxide biosynthetic process (GO:0050665)hydrogen peroxide biosynthetic process (GO:0050665)hydrogen peroxide biosynthetic process (GO:0050665)hydrogen peroxide biosynthetic process (GO:0050665)hydrogen peroxide biosynthetic process (GO:0050665)oxidoreductase activity (GO:0016491)peroxidase activity (GO:0004601)plasma membrane (GO:0005886)plasma membrane (GO:0005886)plasma membrane (GO:0005886)plasma membrane (GO:0005886)positive regulation of cell motility (GO:2000147)positive regulation of defense response to bacterium (GO:1900426)positive regulation of interleukin-8 production (GO:0032757)positive regulation of wound healing (GO:0090303)positive regulation of wound healing (GO:0090303)protein binding (GO:0005515)protein heterodimerization activity (GO:0046982)protein heterodimerization activity (GO:0046982)regulation of thyroid hormone generation (GO:2000609)response to cAMP (GO:0051591)response to cAMP (GO:0051591)response to cAMP (GO:0051591)response to oxidative stress (GO:0006979)superoxide anion generation (GO:0042554)superoxide anion generation (GO:0042554)superoxide-generating NAD(P)H oxidase activity (GO:0016175)thyroid hormone generation (GO:0006590)
Expression (TPM)
DUOX1 — as a Regulated Gene

TFs regulating DUOX1 0 TFs

Transcription factors with Perturb-seq knockdown data for DUOX1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DUOX1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

Data: Effect:
TF Mean coef Binding Outlier TF→Gene link

Elements linked to DUOX1

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DUOX1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility Element Dist. to TSS Link type TFs
chr15:45,022,392–45,023,983 106.9 kb Distal (>10kb) Multiome 755
chr15:45,035,231–45,035,688 94.6 kb Distal (>10kb) Multiome 12
chr15:45,048,481–45,049,541 81.0 kb Distal (>10kb) Multiome 334
chr15:45,117,740–45,118,422 11.8 kb Distal (>10kb) Multiome 509
chr15:45,129,270–45,130,268 at TSS At TSS 118
chr15:45,135,258–45,135,607 5.3 kb Proximal (<10kb) 54
chr15:45,136,533–45,136,691 6.5 kb Proximal (<10kb) 35
chr15:45,162,761–45,163,531 33.2 kb Distal (>10kb) Multiome 392
chr15:45,166,561–45,167,807 37.4 kb Distal (>10kb) Multiome 718
chr15:45,186,761–45,188,736 58.1 kb Distal (>10kb) Multiome 506
chr15:45,198,102–45,201,508 70.5 kb Distal (>10kb) Multiome 1170
chr15:45,224,322–45,226,044 94.9 kb Distal (>10kb) Multiome 242
chr15:45,278,955–45,279,690 149.3 kb Distal (>10kb) Multiome 613
chr15:45,376,152–45,377,230 246.6 kb Distal (>10kb) Multiome 97
chr15:45,378,041–45,378,887 248.5 kb Distal (>10kb) Multiome 372
chr15:45,401,783–45,403,544 272.4 kb Distal (>10kb) Multiome 903

Genome Browser

Genomic view of the DUOX1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.

chr15:45,012,392 – 45,413,544
Proximal 1 kb Distal 10 kb Multiome HiCAR ATAC-seq RNA-seq