This gene encodes a member of the plakin protein family of adhesion junction plaque proteins. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the full-length nature of some variants has not been defined. It has been reported that some isoforms are expressed in neural and muscle tissue, anchoring neural intermediate filaments to the actin cytoskeleton, and some isoforms are expressed in epithelial tissue, anchoring keratin-containing intermediate filaments to hemidesmosomes. Consistent with the expression, mice defective for this gene show skin blistering and neurodegeneration. [provided by RefSeq, Mar 2010]
Transcription factors with Perturb-seq knockdown data for DST. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DST upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DST, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:56,246,560–56,248,146 | 604.4 kb | Distal (>10kb) Multiome | 408 | |
| chr6:56,542,343–56,543,480 | 309.0 kb | Distal (>10kb) Multiome | 961 | |
| chr6:56,842,688–56,844,659 | 7.9 kb | Proximal (<10kb) Multiome | 779 | |
| chr6:56,851,156–56,852,284 | 8 bp | At TSS Multiome | 359 | |
| chr6:56,853,712–56,853,940 | 1.8 kb | Proximal (<10kb) | 37 | |
| chr6:56,854,089–56,854,420 | 2.2 kb | Proximal (<10kb) | 39 | |
| chr6:56,953,824–56,956,167 | 103.3 kb | Distal (>10kb) Multiome | 903 | |
| chr6:57,046,297–57,047,003 | 194.7 kb | Distal (>10kb) Multiome | 808 | |
| chr6:57,089,489–57,090,518 | 238.1 kb | Distal (>10kb) Multiome | 913 |
Genomic view of the DST locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.