Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. Human DPM1 lacks a carboxy-terminal transmembrane domain and signal sequence and is regulated by DPM2. Mutations in this gene are associated with congenital disorder of glycosylation type Ie. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2015]
Transcription factors with Perturb-seq knockdown data for DPM1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DPM1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DPM1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr20:50,730,245–50,732,382 | 227.3 kb | Distal (>10kb) Multiome | 1014 | |
| chr20:50,767,314–50,767,756 | 191.0 kb | Distal (>10kb) Multiome | 140 | |
| chr20:50,790,529–50,791,153 | 167.7 kb | Distal (>10kb) Multiome | 980 | |
| chr20:50,794,408–50,795,648 | 163.6 kb | Distal (>10kb) Multiome | 794 | |
| chr20:50,818,246–50,818,886 | 140.0 kb | Distal (>10kb) Multiome | 844 | |
| chr20:50,870,405–50,871,527 | 87.6 kb | Distal (>10kb) Multiome | 254 | |
| chr20:50,930,214–50,932,320 | 26.9 kb | Distal (>10kb) Multiome | 1082 | |
| chr20:50,958,262–50,959,227 | 143 bp | At TSS Multiome | 929 | |
| chr20:50,992,251–50,993,606 | 34.6 kb | Distal (>10kb) Multiome | 409 | |
| chr20:51,022,526–51,023,913 | 64.7 kb | Distal (>10kb) Multiome | 437 | |
| chr20:51,091,663–51,092,800 | 133.5 kb | Distal (>10kb) Multiome | 122 | |
| chr20:51,221,628–51,222,684 | 263.6 kb | Distal (>10kb) Multiome | 366 | |
| chr20:51,373,737–51,374,717 | 415.7 kb | Distal (>10kb) Multiome HiCAR | 259 | |
| chr20:51,542,047–51,542,945 | 584.2 kb | Distal (>10kb) Multiome HiCAR | 490 |
Genomic view of the DPM1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.